Ethynyl uridine
Web2′-Deoxyuridine (dU) is frequently halogenated to create thymidine analogs useful for studies of DNA synthesis and degradation mechanisms. Derivatized 2′-deoxyuridines used as labeling substrates include chloro-2′-deoxyuridine (CldU), bromodeoxyuridine (BrdU) and/or iododeoxyuridine (IdU). Other useful analogs of 2′-deoxyuridine include ... Web5-Ethynyl uridine (EU) is a novel alternative for bromo uridine (BrU or BrUTP) and can be used to measure RNA synthesis in proliferating cells. EU can be efficiently incorporated …
Ethynyl uridine
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WebUridine found in: Uridine, Uridine, Uridine, Uridine 5'-diphosphoxylose sodium salt, 5-Ethynyl uridine >=98%, SIGMA Uridine 5'-monophosphate 98-100%,.. ... 5-Ethynyl uridine >=98%. MilliporeSigma. Synonyms: 5-EU; EU Molecular Formula: C11H12N2O6 Molecular Weight: 268.22 Linear Structural Formula: C11H12N2O6 Purity: >=98% … Web5-Ethynyl-2′-deoxyuridine (EdU) is a thymidine analog that can be incorporated into replicating DNA for detection of cell proliferation. [ 1] It has an alkyne handle that can ligate with azide-containing fluorescent probes through a highly efficient click chemistry reaction. [ 2] [ 3] EdU nucleoside can be incorporated into the DNA of cells ...
Web5-Ethynyl-uridine has an advantage over its analog, 5-bromo-uridine, because azide-containing dyes are very small in size and exhibit better membrane permeability … WebL. Gutiérrez, ... J.M. de la Fuente, in Comprehensive Medicinal Chemistry III, 2024 1.09.4.1.5 EdU proliferation assay. 5-ethynyl-2′-deoxyuridine (EdU) is a nucleoside …
Web5-Ethynyluridine C11H12N2O6 CID 14885960 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities ... Web5-Ethynyl-2’-uridine 5’-triphosphate, Sodium salt; 5-EUTP; Biological description. Nucleotide analog. Incorporated into RNA by T7 RNA polymerase-mediated in vitro transcription to produce alkyne-functionalized RNA. Purity > 95% Chemical structure. Properties. Molecular weight. 507.97.
WebApr 14, 2024 · 5-Ethynyl Uridine (EU) 作者:齐岳 发布时间:2024-04-14 09:37:05 点击数: 产品名称:5-Ethynyl Uridine (EU)
WebApr 10, 2024 · TEMPOmap begins with metabolically labeling cultured cells with 5-ethynyl uridine (5-EU) 13,15, which adds a bioorthogonal chemical handle on the labeled mRNAs (Fig. 1b). psp apple themeWebDec 26, 2024 · We incubated each RNA (10 nM) in the reconstituted translation reaction solution containing 1.1 mM ethynyl-labelled deoxyribonucleotide, 5-ethynyl-2′-deoxyuridine 5′-triphosphate (EdUTP), and 0.11 mM uridine-5′-triphosphate (UTP). Here, we used a deoxyuridine analog, not a natural deoxyribonucleotide, to evaluate the effect of the lack … psp and tauhorseshoe sets walmartWeb5-Ethynyluridine is a cell-permeable uridine analog. 5-Ethynyluridine can be used in vitro and in vivo to label newly synthesized RNA. 5-Ethynyluridine can be used to study … horseshoe set up specsWeb5-Ethynyl uridine (5-EU) is an alkyne-containing metabolic labeling reagent used to detect global RNA transcription temporally and spatially in both in-vitro and in-vivo with the … horseshoe sets near meWebFeb 16, 2024 · Bar = 10 µm. Ethynyl uridine-containing RNA accumulates in the nucleolus. (A) Arabidopsis seedlings expressing fibrillarin-YFP (magenta) were labeled … horseshoe set near meWebFeb 4, 2024 · In brief, mice were intraperitoneally injected with 0.5 mg alkyne-labeled 5-ethynyl uridine (5-EU; ThermoFisher, Cat# E10345) which readily incorporates into nascent RNA. Titration of 5-EU was done to ensure signal saturation in all BM lineages at the injected dose (Figure S2 A). Mice were sacrificed one hour later after which BM was … horseshoe setup and rules